sc 293 goat hrp Search Results


goat anti-rabbit immunoglobulin (ig) g-horseradish peroxidase (hrp) (sc-2030  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology goat anti-rabbit immunoglobulin (ig) g-horseradish peroxidase (hrp) (sc-2030
Goat Anti Rabbit Immunoglobulin (Ig) G Horseradish Peroxidase (Hrp) (Sc 2030, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+293+goat+hrp/Western+Blotting+Luminol+Reagent/pm31109132-209-12-33
Average 96 stars, based on 1 article reviews
goat anti-rabbit immunoglobulin (ig) g-horseradish peroxidase (hrp) (sc-2030 - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
anti rabbit immunoglobulin g horeseradish peroxidase  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti rabbit immunoglobulin g horeseradish peroxidase
Anti Rabbit Immunoglobulin G Horeseradish Peroxidase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+293+goat+hrp/goat+anti-rabbit+IgG-B/10__1128_slash_iai__71__11__6591___6606__2003-153-19-24
Average 96 stars, based on 1 article reviews
anti rabbit immunoglobulin g horeseradish peroxidase - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
goat anti mouse hrp  (Bio-Rad)
99
Bio-Rad goat anti mouse hrp
Goat Anti Mouse Hrp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+293+goat+hrp/Goat+Anti-Mouse+IgG+(H+%2B+L)-HRP+Conjugate/pmc04036351-177-31-9
Average 99 stars, based on 1 article reviews
goat anti mouse hrp - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
hsp70  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology hsp70
Figure 5. Characterization of the FerroChip in separating exosome-like nanoparticles from biological samples. a We chose to use a 1 µL min-1 sample flow rate in order to separate exosome-like nanoparticles (30 – 150 nm in diameter) from extracellular media and human serum. This sample flow rate maximized the separation between exosome-like nanoparticles and large EVs (200 – 1000 nm in diameter). The ratio of sample flow and sheath flow was 1:5 (sample – 1 µL min-1; sheath –5 µL min-1). Other device and operating parameters remained the same as in previous figures. b Super-resolution microscopic images of exosome-like nanoparticles (PKH 67 green fluorescence) and large EVs (PKH 26 red fluorescence) from the mixture (pre-separation), and the samples after FerroChip processing (large EVs and exosome outlets). A total of 730 particles were analyzed. c Western blot analysis of CD63 and <t>HSP70</t> protein levels in large EVs and exosome-like nanoparticles that were processed by the FerroChip. d Transmission electron microscopy (TEM) images of exosome-like nanoparticles collected from the FerroChip. e Size distribution of separated exosome-like nanoparticles from TEM images (n=755). f Immunofluorescence images of separated exosome-like nanoparticles from human serum. Three channels were used in immunofluorescent staining, including EpCAM (green), CD24 (red), and CD63 (cyan). g Venn diagram depicts the percentage of antibody presence on the surfaces of separated exosome-like nanoparticles (n=12000) from human serum. EpCAM+ alone: 5%; CD24+ alone: 52%; CD63+ alone: 21%; EpCAM+ and CD24+:4%; EpCAM+ / CD63+: 2%; CD24+ / CD63+: 14%; CD63+ / CD24+ / EpCAM+: 2%.
Hsp70, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+293+goat+hrp/HSP70/10__1039_slash_d0lc00609b-239-13-14
Average 96 stars, based on 1 article reviews
hsp70 - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
goat anti rat hrp sc2006  (Santa Cruz Biotechnology)
98
Santa Cruz Biotechnology goat anti rat hrp sc2006
Figure 5. Characterization of the FerroChip in separating exosome-like nanoparticles from biological samples. a We chose to use a 1 µL min-1 sample flow rate in order to separate exosome-like nanoparticles (30 – 150 nm in diameter) from extracellular media and human serum. This sample flow rate maximized the separation between exosome-like nanoparticles and large EVs (200 – 1000 nm in diameter). The ratio of sample flow and sheath flow was 1:5 (sample – 1 µL min-1; sheath –5 µL min-1). Other device and operating parameters remained the same as in previous figures. b Super-resolution microscopic images of exosome-like nanoparticles (PKH 67 green fluorescence) and large EVs (PKH 26 red fluorescence) from the mixture (pre-separation), and the samples after FerroChip processing (large EVs and exosome outlets). A total of 730 particles were analyzed. c Western blot analysis of CD63 and <t>HSP70</t> protein levels in large EVs and exosome-like nanoparticles that were processed by the FerroChip. d Transmission electron microscopy (TEM) images of exosome-like nanoparticles collected from the FerroChip. e Size distribution of separated exosome-like nanoparticles from TEM images (n=755). f Immunofluorescence images of separated exosome-like nanoparticles from human serum. Three channels were used in immunofluorescent staining, including EpCAM (green), CD24 (red), and CD63 (cyan). g Venn diagram depicts the percentage of antibody presence on the surfaces of separated exosome-like nanoparticles (n=12000) from human serum. EpCAM+ alone: 5%; CD24+ alone: 52%; CD63+ alone: 21%; EpCAM+ and CD24+:4%; EpCAM+ / CD63+: 2%; CD24+ / CD63+: 14%; CD63+ / CD24+ / EpCAM+: 2%.
Goat Anti Rat Hrp Sc2006, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+293+goat+hrp/donkey+anti-goat+IgG-B/pm31121182-81-16-36
Average 98 stars, based on 1 article reviews
goat anti rat hrp sc2006 - by Bioz Stars, 2026-09
98/100 stars
  Buy from Supplier
hrp conjugated goat antihuman igg  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology hrp conjugated goat antihuman igg
Figure 5. Characterization of the FerroChip in separating exosome-like nanoparticles from biological samples. a We chose to use a 1 µL min-1 sample flow rate in order to separate exosome-like nanoparticles (30 – 150 nm in diameter) from extracellular media and human serum. This sample flow rate maximized the separation between exosome-like nanoparticles and large EVs (200 – 1000 nm in diameter). The ratio of sample flow and sheath flow was 1:5 (sample – 1 µL min-1; sheath –5 µL min-1). Other device and operating parameters remained the same as in previous figures. b Super-resolution microscopic images of exosome-like nanoparticles (PKH 67 green fluorescence) and large EVs (PKH 26 red fluorescence) from the mixture (pre-separation), and the samples after FerroChip processing (large EVs and exosome outlets). A total of 730 particles were analyzed. c Western blot analysis of CD63 and <t>HSP70</t> protein levels in large EVs and exosome-like nanoparticles that were processed by the FerroChip. d Transmission electron microscopy (TEM) images of exosome-like nanoparticles collected from the FerroChip. e Size distribution of separated exosome-like nanoparticles from TEM images (n=755). f Immunofluorescence images of separated exosome-like nanoparticles from human serum. Three channels were used in immunofluorescent staining, including EpCAM (green), CD24 (red), and CD63 (cyan). g Venn diagram depicts the percentage of antibody presence on the surfaces of separated exosome-like nanoparticles (n=12000) from human serum. EpCAM+ alone: 5%; CD24+ alone: 52%; CD63+ alone: 21%; EpCAM+ and CD24+:4%; EpCAM+ / CD63+: 2%; CD24+ / CD63+: 14%; CD63+ / CD24+ / EpCAM+: 2%.
Hrp Conjugated Goat Antihuman Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+293+goat+hrp/goat+anti-mouse+IgG-HRP/pm40164599-376-32-36
Average 96 stars, based on 1 article reviews
hrp conjugated goat antihuman igg - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
horseradish peroxidase conjugated donkey anti goat antibody  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology horseradish peroxidase conjugated donkey anti goat antibody
Figure 5. Characterization of the FerroChip in separating exosome-like nanoparticles from biological samples. a We chose to use a 1 µL min-1 sample flow rate in order to separate exosome-like nanoparticles (30 – 150 nm in diameter) from extracellular media and human serum. This sample flow rate maximized the separation between exosome-like nanoparticles and large EVs (200 – 1000 nm in diameter). The ratio of sample flow and sheath flow was 1:5 (sample – 1 µL min-1; sheath –5 µL min-1). Other device and operating parameters remained the same as in previous figures. b Super-resolution microscopic images of exosome-like nanoparticles (PKH 67 green fluorescence) and large EVs (PKH 26 red fluorescence) from the mixture (pre-separation), and the samples after FerroChip processing (large EVs and exosome outlets). A total of 730 particles were analyzed. c Western blot analysis of CD63 and <t>HSP70</t> protein levels in large EVs and exosome-like nanoparticles that were processed by the FerroChip. d Transmission electron microscopy (TEM) images of exosome-like nanoparticles collected from the FerroChip. e Size distribution of separated exosome-like nanoparticles from TEM images (n=755). f Immunofluorescence images of separated exosome-like nanoparticles from human serum. Three channels were used in immunofluorescent staining, including EpCAM (green), CD24 (red), and CD63 (cyan). g Venn diagram depicts the percentage of antibody presence on the surfaces of separated exosome-like nanoparticles (n=12000) from human serum. EpCAM+ alone: 5%; CD24+ alone: 52%; CD63+ alone: 21%; EpCAM+ and CD24+:4%; EpCAM+ / CD63+: 2%; CD24+ / CD63+: 14%; CD63+ / CD24+ / EpCAM+: 2%.
Horseradish Peroxidase Conjugated Donkey Anti Goat Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+293+goat+hrp/donkey+anti-goat+IgG-HRP/pmc00085961-210-50-31
Average 96 stars, based on 1 article reviews
horseradish peroxidase conjugated donkey anti goat antibody - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
horseradish peroxidase conjugated goat anti rabbit  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology horseradish peroxidase conjugated goat anti rabbit
Figure 5. Characterization of the FerroChip in separating exosome-like nanoparticles from biological samples. a We chose to use a 1 µL min-1 sample flow rate in order to separate exosome-like nanoparticles (30 – 150 nm in diameter) from extracellular media and human serum. This sample flow rate maximized the separation between exosome-like nanoparticles and large EVs (200 – 1000 nm in diameter). The ratio of sample flow and sheath flow was 1:5 (sample – 1 µL min-1; sheath –5 µL min-1). Other device and operating parameters remained the same as in previous figures. b Super-resolution microscopic images of exosome-like nanoparticles (PKH 67 green fluorescence) and large EVs (PKH 26 red fluorescence) from the mixture (pre-separation), and the samples after FerroChip processing (large EVs and exosome outlets). A total of 730 particles were analyzed. c Western blot analysis of CD63 and <t>HSP70</t> protein levels in large EVs and exosome-like nanoparticles that were processed by the FerroChip. d Transmission electron microscopy (TEM) images of exosome-like nanoparticles collected from the FerroChip. e Size distribution of separated exosome-like nanoparticles from TEM images (n=755). f Immunofluorescence images of separated exosome-like nanoparticles from human serum. Three channels were used in immunofluorescent staining, including EpCAM (green), CD24 (red), and CD63 (cyan). g Venn diagram depicts the percentage of antibody presence on the surfaces of separated exosome-like nanoparticles (n=12000) from human serum. EpCAM+ alone: 5%; CD24+ alone: 52%; CD63+ alone: 21%; EpCAM+ and CD24+:4%; EpCAM+ / CD63+: 2%; CD24+ / CD63+: 14%; CD63+ / CD24+ / EpCAM+: 2%.
Horseradish Peroxidase Conjugated Goat Anti Rabbit, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+293+goat+hrp/goat+anti-rabbit+IgG-HRP/pmc01797491-114-42-54
Average 96 stars, based on 1 article reviews
horseradish peroxidase conjugated goat anti rabbit - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
horseradish peroxidase hrp conjugated goat anti mouse igg  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology horseradish peroxidase hrp conjugated goat anti mouse igg
Figure 5. Characterization of the FerroChip in separating exosome-like nanoparticles from biological samples. a We chose to use a 1 µL min-1 sample flow rate in order to separate exosome-like nanoparticles (30 – 150 nm in diameter) from extracellular media and human serum. This sample flow rate maximized the separation between exosome-like nanoparticles and large EVs (200 – 1000 nm in diameter). The ratio of sample flow and sheath flow was 1:5 (sample – 1 µL min-1; sheath –5 µL min-1). Other device and operating parameters remained the same as in previous figures. b Super-resolution microscopic images of exosome-like nanoparticles (PKH 67 green fluorescence) and large EVs (PKH 26 red fluorescence) from the mixture (pre-separation), and the samples after FerroChip processing (large EVs and exosome outlets). A total of 730 particles were analyzed. c Western blot analysis of CD63 and <t>HSP70</t> protein levels in large EVs and exosome-like nanoparticles that were processed by the FerroChip. d Transmission electron microscopy (TEM) images of exosome-like nanoparticles collected from the FerroChip. e Size distribution of separated exosome-like nanoparticles from TEM images (n=755). f Immunofluorescence images of separated exosome-like nanoparticles from human serum. Three channels were used in immunofluorescent staining, including EpCAM (green), CD24 (red), and CD63 (cyan). g Venn diagram depicts the percentage of antibody presence on the surfaces of separated exosome-like nanoparticles (n=12000) from human serum. EpCAM+ alone: 5%; CD24+ alone: 52%; CD63+ alone: 21%; EpCAM+ and CD24+:4%; EpCAM+ / CD63+: 2%; CD24+ / CD63+: 14%; CD63+ / CD24+ / EpCAM+: 2%.
Horseradish Peroxidase Hrp Conjugated Goat Anti Mouse Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+293+goat+hrp/m-IgG%CE%BA+BP-HRP/pmc08351703-177-55-37
Average 96 stars, based on 1 article reviews
horseradish peroxidase hrp conjugated goat anti mouse igg - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
anti rabbit igg horseradish peroxidase conjugate  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti rabbit igg horseradish peroxidase conjugate
Figure 5. Characterization of the FerroChip in separating exosome-like nanoparticles from biological samples. a We chose to use a 1 µL min-1 sample flow rate in order to separate exosome-like nanoparticles (30 – 150 nm in diameter) from extracellular media and human serum. This sample flow rate maximized the separation between exosome-like nanoparticles and large EVs (200 – 1000 nm in diameter). The ratio of sample flow and sheath flow was 1:5 (sample – 1 µL min-1; sheath –5 µL min-1). Other device and operating parameters remained the same as in previous figures. b Super-resolution microscopic images of exosome-like nanoparticles (PKH 67 green fluorescence) and large EVs (PKH 26 red fluorescence) from the mixture (pre-separation), and the samples after FerroChip processing (large EVs and exosome outlets). A total of 730 particles were analyzed. c Western blot analysis of CD63 and <t>HSP70</t> protein levels in large EVs and exosome-like nanoparticles that were processed by the FerroChip. d Transmission electron microscopy (TEM) images of exosome-like nanoparticles collected from the FerroChip. e Size distribution of separated exosome-like nanoparticles from TEM images (n=755). f Immunofluorescence images of separated exosome-like nanoparticles from human serum. Three channels were used in immunofluorescent staining, including EpCAM (green), CD24 (red), and CD63 (cyan). g Venn diagram depicts the percentage of antibody presence on the surfaces of separated exosome-like nanoparticles (n=12000) from human serum. EpCAM+ alone: 5%; CD24+ alone: 52%; CD63+ alone: 21%; EpCAM+ and CD24+:4%; EpCAM+ / CD63+: 2%; CD24+ / CD63+: 14%; CD63+ / CD24+ / EpCAM+: 2%.
Anti Rabbit Igg Horseradish Peroxidase Conjugate, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+293+goat+hrp/rabbit+anti-goat+IgG-HRP/pmc01198224-46-22-12
Average 96 stars, based on 1 article reviews
anti rabbit igg horseradish peroxidase conjugate - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier
phosphorylated vasodilator stimulated phosphoprotein  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology phosphorylated vasodilator stimulated phosphoprotein
Figure 5. Characterization of the FerroChip in separating exosome-like nanoparticles from biological samples. a We chose to use a 1 µL min-1 sample flow rate in order to separate exosome-like nanoparticles (30 – 150 nm in diameter) from extracellular media and human serum. This sample flow rate maximized the separation between exosome-like nanoparticles and large EVs (200 – 1000 nm in diameter). The ratio of sample flow and sheath flow was 1:5 (sample – 1 µL min-1; sheath –5 µL min-1). Other device and operating parameters remained the same as in previous figures. b Super-resolution microscopic images of exosome-like nanoparticles (PKH 67 green fluorescence) and large EVs (PKH 26 red fluorescence) from the mixture (pre-separation), and the samples after FerroChip processing (large EVs and exosome outlets). A total of 730 particles were analyzed. c Western blot analysis of CD63 and <t>HSP70</t> protein levels in large EVs and exosome-like nanoparticles that were processed by the FerroChip. d Transmission electron microscopy (TEM) images of exosome-like nanoparticles collected from the FerroChip. e Size distribution of separated exosome-like nanoparticles from TEM images (n=755). f Immunofluorescence images of separated exosome-like nanoparticles from human serum. Three channels were used in immunofluorescent staining, including EpCAM (green), CD24 (red), and CD63 (cyan). g Venn diagram depicts the percentage of antibody presence on the surfaces of separated exosome-like nanoparticles (n=12000) from human serum. EpCAM+ alone: 5%; CD24+ alone: 52%; CD63+ alone: 21%; EpCAM+ and CD24+:4%; EpCAM+ / CD63+: 2%; CD24+ / CD63+: 14%; CD63+ / CD24+ / EpCAM+: 2%.
Phosphorylated Vasodilator Stimulated Phosphoprotein, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+293+goat+hrp/bovine+anti-goat+IgG-HRP/pmc04487134-45-6-31
Average 94 stars, based on 1 article reviews
phosphorylated vasodilator stimulated phosphoprotein - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier
horse radish peroxidase coupled goat  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology horse radish peroxidase coupled goat
Figure 5. Characterization of the FerroChip in separating exosome-like nanoparticles from biological samples. a We chose to use a 1 µL min-1 sample flow rate in order to separate exosome-like nanoparticles (30 – 150 nm in diameter) from extracellular media and human serum. This sample flow rate maximized the separation between exosome-like nanoparticles and large EVs (200 – 1000 nm in diameter). The ratio of sample flow and sheath flow was 1:5 (sample – 1 µL min-1; sheath –5 µL min-1). Other device and operating parameters remained the same as in previous figures. b Super-resolution microscopic images of exosome-like nanoparticles (PKH 67 green fluorescence) and large EVs (PKH 26 red fluorescence) from the mixture (pre-separation), and the samples after FerroChip processing (large EVs and exosome outlets). A total of 730 particles were analyzed. c Western blot analysis of CD63 and <t>HSP70</t> protein levels in large EVs and exosome-like nanoparticles that were processed by the FerroChip. d Transmission electron microscopy (TEM) images of exosome-like nanoparticles collected from the FerroChip. e Size distribution of separated exosome-like nanoparticles from TEM images (n=755). f Immunofluorescence images of separated exosome-like nanoparticles from human serum. Three channels were used in immunofluorescent staining, including EpCAM (green), CD24 (red), and CD63 (cyan). g Venn diagram depicts the percentage of antibody presence on the surfaces of separated exosome-like nanoparticles (n=12000) from human serum. EpCAM+ alone: 5%; CD24+ alone: 52%; CD63+ alone: 21%; EpCAM+ and CD24+:4%; EpCAM+ / CD63+: 2%; CD24+ / CD63+: 14%; CD63+ / CD24+ / EpCAM+: 2%.
Horse Radish Peroxidase Coupled Goat, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sc+293+goat+hrp/Horseradish+peroxidase+(HRP)%2C+RZ+1%2E0/pm24916378-249-0-25
Average 96 stars, based on 1 article reviews
horse radish peroxidase coupled goat - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier

Image Search Results


Figure 5. Characterization of the FerroChip in separating exosome-like nanoparticles from biological samples. a We chose to use a 1 µL min-1 sample flow rate in order to separate exosome-like nanoparticles (30 – 150 nm in diameter) from extracellular media and human serum. This sample flow rate maximized the separation between exosome-like nanoparticles and large EVs (200 – 1000 nm in diameter). The ratio of sample flow and sheath flow was 1:5 (sample – 1 µL min-1; sheath –5 µL min-1). Other device and operating parameters remained the same as in previous figures. b Super-resolution microscopic images of exosome-like nanoparticles (PKH 67 green fluorescence) and large EVs (PKH 26 red fluorescence) from the mixture (pre-separation), and the samples after FerroChip processing (large EVs and exosome outlets). A total of 730 particles were analyzed. c Western blot analysis of CD63 and HSP70 protein levels in large EVs and exosome-like nanoparticles that were processed by the FerroChip. d Transmission electron microscopy (TEM) images of exosome-like nanoparticles collected from the FerroChip. e Size distribution of separated exosome-like nanoparticles from TEM images (n=755). f Immunofluorescence images of separated exosome-like nanoparticles from human serum. Three channels were used in immunofluorescent staining, including EpCAM (green), CD24 (red), and CD63 (cyan). g Venn diagram depicts the percentage of antibody presence on the surfaces of separated exosome-like nanoparticles (n=12000) from human serum. EpCAM+ alone: 5%; CD24+ alone: 52%; CD63+ alone: 21%; EpCAM+ and CD24+:4%; EpCAM+ / CD63+: 2%; CD24+ / CD63+: 14%; CD63+ / CD24+ / EpCAM+: 2%.

Journal: Lab on a Chip

Article Title: Label-free ferrohydrodynamic separation of exosome-like nanoparticles

doi: 10.1039/d0lc00609b

Figure Lengend Snippet: Figure 5. Characterization of the FerroChip in separating exosome-like nanoparticles from biological samples. a We chose to use a 1 µL min-1 sample flow rate in order to separate exosome-like nanoparticles (30 – 150 nm in diameter) from extracellular media and human serum. This sample flow rate maximized the separation between exosome-like nanoparticles and large EVs (200 – 1000 nm in diameter). The ratio of sample flow and sheath flow was 1:5 (sample – 1 µL min-1; sheath –5 µL min-1). Other device and operating parameters remained the same as in previous figures. b Super-resolution microscopic images of exosome-like nanoparticles (PKH 67 green fluorescence) and large EVs (PKH 26 red fluorescence) from the mixture (pre-separation), and the samples after FerroChip processing (large EVs and exosome outlets). A total of 730 particles were analyzed. c Western blot analysis of CD63 and HSP70 protein levels in large EVs and exosome-like nanoparticles that were processed by the FerroChip. d Transmission electron microscopy (TEM) images of exosome-like nanoparticles collected from the FerroChip. e Size distribution of separated exosome-like nanoparticles from TEM images (n=755). f Immunofluorescence images of separated exosome-like nanoparticles from human serum. Three channels were used in immunofluorescent staining, including EpCAM (green), CD24 (red), and CD63 (cyan). g Venn diagram depicts the percentage of antibody presence on the surfaces of separated exosome-like nanoparticles (n=12000) from human serum. EpCAM+ alone: 5%; CD24+ alone: 52%; CD63+ alone: 21%; EpCAM+ and CD24+:4%; EpCAM+ / CD63+: 2%; CD24+ / CD63+: 14%; CD63+ / CD24+ / EpCAM+: 2%.

Article Snippet: The membrane was then incubated overnight with exosome primary antibodies against CD63 and HSP70 (Santa Cruz Biotechnology, Inc., Dallas, TX following incubation with Goat-Rabbit-HRP secondary antibody (Santa Cruz Biotechnology, Inc., Dallas, TX).

Techniques: Fluorescence, Western Blot, Transmission Assay, Electron Microscopy, Immunofluorescence, Staining